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ATCC a549
A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a549+cells/A549/custom%40crm-ccl-185%4010%2E64898%2F2026%2E08%2E02%2E742305
Average 99 stars, based on 9081 article reviews

a549 - by Bioz Stars, 2026-09

99/100 stars

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Recombinant:

Article Title: AI-Enforced Ultra-Large Virtual Screening Discovers Potent CD28 Binders.
Article Snippet: .. Tumor spheroids were established by plating A549 cells (from ATCC) into ultralow attachment 96-well plates and allowing aggregation for 48 h. Spheroids were subsequently primed with recombinant human interferon-γ (50 ng/mL) for 24 h prior to immune cell introduction. .. Cryopreserved human peripheral blood mononuclear cells (PBMCs) from STEMCELL Technologies were thawed and added to the spheroids at an effector-to-target ratio of 5:1.

Cell Culture:

Article Title: When TiO 2 meets pharmaceuticals: Photocatalytic degradation and environmental safety unveiled.
Article Snippet: .. A549 cells (ATCC CCL− 185, Manassas, VA, USA) were cultured under standard conditions according to ATCC recommendations. ..

Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model
Article Snippet: Adenocarcinoma human alveolar basal epithelial cells line: A549 (CRM-CCL-185), human colorectal adenocarcinoma cell line (HTB-38), and C57/BL6 background murine Lewis lung carcinoma cell line LL/2 (LLC1) (CRL-1642) were purchased from American Type Culture Collection (ATCC). .. A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco). .. All media used were supplemented with 10% fetal bovine serum (Sigma) and 1% penicillin/streptomycin (Invitrogen).

Article Title: Clinical significance of LINC01093 in children with severe pneumonia and its regulation of miR‑326/MAPK1 axis.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Cell cultivation and model construction The MRC-5 and A549 cells were obtained from the American Type Culture Collection (USA) and cultured in DMEM medium (Gibco, USA) containing 1% penicillin-streptomycin and 10% fetal bovine serum (FBS) at 37°C and 5% CO2. ..

Modification:

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$Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.$

Journal: Advances in Radiation Oncology

Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model

doi: 10.1016/j.adro.2026.102051

Figure Lengend Snippet: Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

Article Snippet: A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco).

Techniques: In Vitro, Growth Assay, Clonogenic Cell Survival Assay, Generated